Procedure:
1. Define chromatography: 2. Gather materials for the lab. 3. Mark the filter paper with a pencil line 2.0 cm from the end of the paper. 4. Write the color of the felt marker in pencil at the top of the filter paper. 5. With a felt pen, make a dot on the middle of the pencil line. Let the spot dry. 6. Repeat the process adding more ink to the spot. Try to make it thicker, not wider. 7. Set that filter paper aside. 8. Repeat #2-5 using a new filter paper and another color. 9. Secure the two pieces of filter paper using the paper clip and hang them so they reach the 25-50 ml mark in the DRY 400 ml beaker. 10. Remove the papers from the DRY beaker and set aside.
11. Using the 10 ml graduated cylinder, measure and pour 2.0 ml of vinegar into the 400 ml beaker. 12. Fill the 400 ml beaker with enough water to make a water-vinegar solution to the 25-50 ml mark. 13. Carefully place the two pieces of filter paper in the beaker so the dots are not touching the water-vinegar solution. If they fall into the solution, you will have to start over. 14. Observe the ink spot as the water-vinegar solution moves up the paper. 15. When the solution reaches the paper clip, remove it and mark the end of the solution with a pencil. 16. Let the filter papers dry on a paper towel. 17. Record your observations. 18. Measure and sketch the colors that you observe.
Analysis: Answer these on another sheet of paper in ink or type. (Rewrite questions.)
The Essay on Osmosis Of Potatoes In Different Sucrose Solutions
The aim of this experiment is to test whether more water moves out of a potato when it is placed in a sweeter sucrose solution than a potato in a less sweet solution. The hypothesis of the experiment is that we expect more water to move out of the potato placed in the sweet solution than the potato placed in a less sweet solution. Independent variable: concentration of sucrose, concentrations: ...
1. Define chromatography. 2. With the black marker, how many different colors can you identify? Describe this. 3. Why do you see different colors at various locations on the filter paper? 4. Compare and contrast the colors in the black and/or brown markers on the filter paper with one other marker that is not black/brown. 5. How is the technique of chromatography used to separate a mixture? 6. Is chromatography a physical or chemical change? Explain!
Conclusion:
1. Describe what you have learned from this lab.
2. Research different types of chromatography to understand how it is used. Rewrite this in your own words.
