I record the time taken for the solution to go from black to colourless. Once colourless it proves there is starch present. I will use a stop clock to measure the time taken. Control variables This is what I will keep the same in the experiment so the test will be fair. I will keep the volume of starch and amylase the same in each part of the experiment. The test tubes will stay in the water for five minutes, and three drops of iodine will go into each test tube of starch. Apparatus 5 Beakers 10 Test tubes 5 Thermometers Starch Amylase Ice Iodine Kettle Warm water Dropping pipette
Graduated cylinder 1 Bunsen burner Tripod Heat proof mat Gauze Method 1. Collect all the needed apparatus needed or the experiment. 2. Set up one water bath at a time, starting with water bath 1. (10 degrees) 3. Fill the beaker up with cold water and add ice, and measure the temperature of the water. 4. Measure out 5cm3 of starch and 3 cm3 of amylase and place into two different test tubes; add three drops of iodine to the starch. 5. Set the test tubes into the water bath and leave for five minutes. Pour the starch and amylase into the water bath. 6. Start the clock.
Record in a table the times for the solution to turn from black to colourless. 8. Do the experiment for all the other temperatures, but adjust the water to the required temperature. 25 degrees- room temperature, 40 degrees, and 55 degrees add hot water and 70 degrees add boiled water from a kettle, if it exceeds required temperature decrease temperature with ice cubes. 9. Repeat the experiment for an average. Prediction and justification Enzymes are protein molecules found in living cells. When an enzyme combines with a substrate in its active, it will begin to break down that specific substrate.
The Essay on Conductivity Of Streams Water Area Temperature
The measure of the capacity of water to allow an electrical current is called conductivity. Conductivity in streams is affected by many factors such as the elements or compounds found in the water, the temperature of the water, the geology of the area, and the pollution in the water. Each of these factors affect the conductiity in some way. Many inorganic dissolved solids increase the conductivity ...
As the enzyme is a catalyst it will speed up the reaction but not take part in the reaction itself, they combine together forming an enzyme substrate complex. Due to the weakened state of the substrate its bonds are broken and new products are made. In my experiment the substrate will be the starch and the enzyme is amylase. The temperature of the solution affects the rate of reaction, the kinetic energy increases as the temperature does (as the temperature increases the molecules begin to collide faster causing more reactions).
