ts, and biochemical pathways. In comparing the organism 60% of M. jannaschii’s coding regions did not correlate with the known sequence database. 40% of the coding regions match the sequences of bacteria and eukaryotes. Those sequences that are similar to bacteria are genes related to energy production, cell division, and metabolism, whereas the transcription, translation, and replication gene sequences seem to be more similar to eukaryotes . *EM/*Methanococcus jannaschii*/EM* consists of three parts: the main circular chromosome, and a large and small circular extrachromosomal element (ECE).. The chromosome contains 1,664,976 base pairs (G+C content 31.4%), the large ECE, 58,407 bp (G+C content 28.2%), and the small ECE, 16,550 bp (G+C content 28.8%).
There are a total of 1738 predicted coding regions:1682 regions on the chromosome, 44 on the large ECE, and 12 on the small ECE.
The function of the ECE’s is The National Center for Genome Resources has published the complete genome of M. jannaschii in its publicly available Genome Sequence DataBase (GSDB).
The sequence of the genome is the work of researchers at The Institute of Genomic Research (TIGR), the University of Illinois, Urbana; and Johns Hopkins University. The complete M. jannaschii genome is available only from GSDB. Other databases break large sequences into pieces no longer than 300 KB. In addition, GSDB allows third-party annotation to M. jannaschii . This enables researchers other than the original authors of the sequence to contribute new information to this genome sequence.
The Term Paper on Genome Sequencing Phylogenetic Classification
Genome Sequencing Microbiology has entered the realm of genome sequencing. This biological revolution is opening up new dimensions in our view of life. In 1995, a report on the entire DNA sequence for the genome of the bacteria Haemophilus influenzae was published. Although the genomes for a number of viruses had been completed before this, H. influenzae was the first free-living organism to have ...
PAN, the proteasome-activating nucleotidase from archaebacteria, is a protein-unfolding molecular chaperone. The proteasome-activating nucleotidase (PAN) from Methanococcus jannaschii is a complex of relative molecular mass 650,000 that is homologous to the ATPases in the eukaryotic 26S proteasome. When mixed with 20S archaeal proteasomes and ATP, PAN stimulates protein degradation. Here we show that PAN reduces aggregation of denatured proteins and enhances their refolding. These processes do not require ATP hydrolysis, although ATP binding enhances the ability of PAN to prevent aggregation. PAN also catalyses the unfolding of the green fluorescent protein with an 11-residue ssrA extension at its carboxy terminus (GFP11).
This unfolding requires ATP hydrolysis, and is linked to GFP11 degradation when 20S proteasomes are also present. This unfolding activity seems to be essential for ATP-dependent proteolysis, although PAN may function by itself as a molecular chaperone. *!——————————-!* Pressure effects on the composition and thermal behavior of lipids from the deep-sea thermophile Department of Chemical Engineering, University of California, Berkeley 94720, USA. The deep-sea archaeon Methanococcus jannaschii was grown at 86 degrees C and under 8, 250, and 500 atm (1 atm = 101.29 kPa) of hyperbaric pressure in a high-pressure, high-temperature bioreactor. The core lipid composition of cultures grown at 250 or 500 atm, as analyzed by supercritical fluid chromatography, exhibited an increased proportion of macrocyclic archaeol and corresponding reductions in aracheol and caldarchaeol compared with the 8-atm cultures. Thermal analysis of a model core-lipid system (23% archaeol, 37% macrocyclic archaeol, and 40% caldarchaeol) using differential scanning calorimetry revealed no well-defined phase transition in the temperature range of 20 to 120 degrees C. Complementary studies of spin-labeled samples under 10 and 500 atm in a special high-pressure, high-temperature electron paramagnetic resonance spectroscopy cell supported the differential scanning calorimetry phase transition data and established that pressure has a lipid-ordering effect over the full range of M. jannaschii’s growth temperatures. Specifically, pressure shifted the temperature dependence of lipid fluidity by ca.
The Term Paper on Kinetic Energy Particles Pressure Gas
Chapter 13- States of Matter Gases Kinetic Molecular Theory. Model to describe properties of gases. Kinetic = move. Theory describes behavior in terms of particles in motion. Model makes assumptions about size, motion, and energy of gas particles Particle size. Gases consist of small particles that are separated from one another by empty space. Because gas particles are far apart, there are no ...
10 degrees C/500 atm.
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