Title: Basic Plant Cells
Purpose: The purpose of this lab is to
1. To identify cell structures using the microscope.
2. To apply a vital stain to living cells.
3. To explain why vital stains are used in Biology.
4. To study the structure {and function} of plant organelles.
Materials:
1. Microscopes
2. Slides
3. Lugols iodine
4. Onion
5. Coverslips
6. Paper towels
Safety
1. Don’t use coarse adjustment on high power.
2. Be careful with Lugols iodine, it will stain you and your cloths.
3. Carry microscopes with two hands.
Procedure:
1. Prepare a wet mount of white onion cells as demonstrated in by your teacher.
2. View this wet mount on low, medium, and high powers before applying stain.
3. Apply Lugols iodine to your onion cell wet mount as demonstrated by your teacher.
4. Make drawings of your stained cells, one under low power and one under medium power and one under high power. Label the cell membrane, cell wall, cytoplasm, and nucleus.
Data: see attached drawing/chart/table/graph.
Questions
1. Why is it necessary to stain onion cells?
It is necessary to stain the onion so you can see the cell more clearly.
The Essay on Fuel Cell Technology Cells Electricity Power
Fuel cell technology was created by Sir William Robert Grove in 1839. It was later established and developed by Ballard Power systems. A fuel cell is a device that produces electricity from a fuel and an oxidizer, a substance that combines with fuel. Fuel cells have a high efficiency level. They are used in boats, trucks, and laptops. To this day, fuel cells are providing the electricity for the ...
2. What stain is used on plants?
The stain that is used is the Lugols iodine.
3. What is responsible for green appearance of the elodea cells?
The thing that is responsible for the green appearance is the chlorophyll
4. What organelle was visible in the elodea cells but not visible in the onion cells?
The organelle that was visible in the elodea cells was the chloroplasts.
5. Explain why the organelle was visible in the elodea cells only?
It was visible because we used iodine which made it clearer.
6. What is the basic shape of a plant cell? How does the shape differ from a typical animal cell?
The basic shape of a plant cell is a rectangle. The shape differs because the plant cell has a rectangular shape and the animal cell has a circular shape.
7. What cell structure{s} identify an organism as an autotroph?
The cell structure that identifies an organism as an autotroph is the mitochondria.
The main organelles visible in a plant cell when using a compound light microscope is the cell membrane, cell wall, cytoplasm, and the nucleus. The function of the cell membrane is it’s a flexible barrier that that goes around the cell. The cell wall is a rigid wall that provides and maintains the shape of these cells. The cytoplasm contains mostly water, but is full of proteins that controls cell metabolism including signal transduction pathways. The nucleus is a double membrane and communicates with the surrounding cytosol via numerous nuclear pores. It is important to have a central large vacuole because it helps store nutrients and waste products , helping increase cell size during growth. One visible difference between the plant and animal cell is they are both shaped differently. The animal cell is circular and the plant cell is rectangular. Another visible difference is the plant cell has a large vacuole but the animal cell does not. The cell wall is a protective barrier and the vacuole helps store nutrients and waste products.
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Duckweeds belong to a family of hydrophytic (aquatic) plants called Lemnaceae and are considered the smallest flowering plants. These plants lack the recognizable stems and leaves and they are instead characterized by several small flattened floating structures, which are believed to be modified leaves, often called as thalloids, with simple or no roots. Duckweed roots do not exhibit secondary ...
