Since we increased the number of discs to increase the concentration, we provided more surface area for the enzyme catalase to break down the hydrogen peroxide. From the results obtained from Group A,B,C and D, shown through the graphs, one can see that as the concentration is increased, as a result of the increase in the number of discs, the rate at which the enzyme breaks down the hydrogen peroxide is increased. Furthermore, it is evident through the increased volume of oxygen gas that is produced as a result of the increased concentration; thus it is evident that the increase in concentration results in the increased rate of reaction.
B) Temperature (Increase or decrease) – It is know that if there is an increase/decrease in temperature then as a result there is an increase or decrease in enzyme activity; thus, the temperature is proportional to the enzyme activity. From the results obtained from Graph E and F, shown through the graph, one can see that in Group E, when the substrate (hydrogen peroxide) was cooled in the water bath to approx 0oC, the rate of oxygen production was reduced by 62mL/minute.
The Essay on Enzyme Controlled Reactions Lab
1)Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive, but is dramatically affected by ...
To explain, the rate of oxygen production was 165mL/minute for the control, but after the temperature of the substrate was decreased the rate of oxygen production dropped to 103mL/minute. In Group F, we see a similar trend in that the rate of oxygen production is increased with the increase in temperature of the hydrogen peroxide. Thus, it is evident in that with the increase or decrease in temperature, the enzyme activity is affected respectively.
C)Varying State of Enzyme – It is known that protein denaturation is any physical or chemical process that changes the protein structure (breaks bonds) and makes it incapable of performing its normal function. In Group G, that is what exactly occurs, as a result of cooking the enzyme, we denature the protein; thus destroying the enzyme. Hence, the enzyme is not present to breakdown the hydrogen peroxide, therefore nothing occurs. It is evident through the data as well, once we found the control which was 153mL/minute, we did the experiment with the denature enzyme, and nothing occurred, therefore the rate was 0mL/minute.
Furthermore, with the change of state, it is evident that the enzyme can no longer function to breakdown the hydrogen peroxide. Sources of Error: 1)Loss of Oxygen – Once the experiment is performed, and the enzyme catalase breaks down the hydrogen peroxide releasing the oxygen, there was released oxygen that was not contained in the graduated cylinder, and leaked through the top (since graduated cylinder is upside down for experiment).
Hence, the obtained rate of oxygen produced is inaccurate, therefore effecting the results. One way to fix this is to obtain a flat surface water bath.
To explain, the water bath our group used was very old and used, the bottom layer seemed to be somewhat malleable; thus, deforming the shape of the bottom. Therefore, once the time of the experiment was up and we returned the graduated cylinder to the upright position, the ‘trenches’ in the water bath allowed some leakage of oxygen 2)Amount of Enzyme on each Disc – When applying the catalase enzyme onto the discs, by human hands it is not possible to apply the same amount of the enzyme to each disc, therefore, each experiment had discs with different amounts of enzyme which ultimately led to unfair and inaccurate results.
The Essay on Natural Increase Rate People High
Identify those areas of the world which have very high rates of natural increase in population today and give reasons for them. Rate of natural increase is defined as the rate at which a population is increasing / decreasing in a given year due to a surplus or deficit of births over deaths, expressed as a percentage of the base population. The birth rate (Br) is the number of live births per 1000 ...
To resolve this issue, we would have to use an instrument that measure the amount excreted out onto each discs, such as a syringe or eye dropper. 3)Cross contamination – When the uneven amount of catalase enzyme is placed onto each discs, for some of the trial, some excess enzyme fell into the hydrogen peroxide causing a side reaction. As a result of this side reaction the rate of oxygen produced is inaccurate because not all of it was collected. To resolve this, there would have to be a way to barricade the enzyme from the hydrogen peroxide, and then remove the barricade when the experiment is taking place.
Conclusion: In conclusion, the hypotheses were correct, and it was not only evident during the lab but from the result as well. For concentration – if there is an increase in enzyme concentration, then there will be an increased rate of reaction as a result of more active sites being available. For Varying Temperature – for groups E and F, if there is an increase in temperature, then there is an increase in the enzyme For Varying – if the enzyme is boiled, then the protein is denatured, therefore no reaction occurs.
