Answer the following questions about the results of this activity. Record your answers in the boxes. Send your completed lab report to your instructor. Dont forget to save your lab report to your computer Activity 1 Record your data from Activity 1 in the boxes below. Enter the data for the sample you used in each trial (5000 rpm, 10000 rpm, etc) in the appropriate columns and the corresponding g-force, number of layers, and position of layers position results. You will need to use the following formula to assist with your laboratory report G-force 0 00001118 x radius of centrifuge arm x (rpm)2 The radius of the centrifuge arm for this instrument is 10 cm. Speed 5000 rpm 10000 rpm 15000 rpm 20000 rpm G-force27951118025155 44720 Number of Layers 2 4 5 3 Position of Layers Top and middle of tube 2 at the top And 2 in the middle/bottomAll layers nearly equidistance from each other from top to bottom 1 layer in top 1 layer in middle The rest on the bottom Explain what happens to the G-force as the speed of the centrifuge increases.
Which is likely the best speed to separate the components of this soil sample Why Describe in which layers you are likely to find the organic matter, gravel, sand, silt, and clay at the following speeds 5000 rpm 15000 rpm Activity 2 Record your data from Activity 2 in the boxes below. Enter the data for the sample you used in each trial (0.5, 1.5, 2.0, and 2.5 grams/ liter cesium chloride) in the appropriate columns with the corresponding g-force, number of layers, and position of layers results. Cesium Chloride grams/liter 0.5 1.5 2.0 2.5 Number of Layers 2 5 5 6 Position of LayersLittle on top rest on bottom2 near top 3 from the middle to the bottom2 near top 3 thinner layers from the middle to the bottom2 thin layers near top 1 layer below that last 3 in same position as before Explain what happens to the drag as the cesium chloride concentration increases. Which is likely the best cesium chloride concentration to separate the components of this soil sample Why Describe in which layers you are likely to find the organic matter, gravel, sand, silt, and clay at the following speeds 0.5 grams/liter of cesium chloride 2.5 grams/liter of cesium chloride Explain any differences in the thickness of similar bands at the different cesium chloride concentrations.
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