MATERIALS AND METHODS
This research study used the process of extraction to test the effectiveness of Acapulco (Cassia Alata) leaves in killing or eradicating the fungi Microsporum canis. The raw material that the researchers used in the experiment is the Acapulco with the scientific name Cassia Alata. It belongs to the family of Leguminosae. It is a shrub which is erected and grew for about 2 meters tall. The leaves of the plant posseses fungicidal, insecticidal and pesticidal properties.
EXTRACTION OF LEAVES
In getting the extract for this study, plants were first collected from rural areas from Red V and Ilayang Talim of Lucena City. The researchers pick those fresh leaves for they are more effective to used. Then the researchers decided to have a measure of 2 kilograms of the Acapulco leaves. The fresh leaves were washed with tap water and cleaned thoroughly with a clean cloth. The leaves were immediately pound by the use of a mortar and pestle. The material was filtered through a clean Muslim cloth and the leaf extract was stored in clean test tubes, with the same size and having the same amount of extract and stored in a refrigerator to preserved it’s freshness.
ANTI-FUNGAL BIOASSAY
There were four set- ups during the experiment. Each of those set up were labeled as set up A,B,C and D having the same amount of fungi each. Set up A was consist of 20 % of the acapulco leaves extract and 80% water. Set up B was consist of 50% of the acapulco leave extract and 50% of water. Set up C was consist of 80% of the Acapulco leaves extract. Set up D which was the control set up having the commercially used product which is the canesten. Each of these set up was observed properly and then the researchers measured the length of time it took for the fungio be destroyed due to the acapulco leaves extract. The mortality rate of each fungi in each set up were observed.
The Term Paper on Mayana Leaves
I. Introduction People of different cultures have long used plants, especially those with therapeutic values. They believe plants to have healing powers. In most parts of our country, many people have embraced the powers of plants in their daily lives. Modern medicine has downplayed the importance of this traditional knowledge, but now it has begun to acknowledge the value of using plants for ...
LABORATORY TEST
The researchers need sample of the fungi “Microsporum canis” for the experiment. The researchers got the sample fungi from Department of Science and Technology in Taguig. They bought a test tube containing fungi “Microsporum canis” and then bring it back to Lucena City. The researchers brought the fungi in the CEFI for the laboratory test and wait for about two weeks for the result.
TESTING THE PROPERTIES OF THE EXTRACT
The researchers get four people that have the infection of Microsporum canis. These people will be the respondents of the researchers for the experiment. The extract in the set up it is used with the first respondent. The extract from set up B is used with the second respondent. The extract from set up C is used in the third respondent. The control variable which is the set up D is then used with the fourth and last respondent. Then the researchers observed the effectiveness of Acapulco leaves in each respondent.