Polymerase chain reaction (PCR) is a technique which is used to amplify the number of copies of a specific region of DNA, in order to produce enough DNA to be adequately tested. This technique can be used to identify with a very high-probability, disease-causing viruses and/or bacteria, a deceased person, or a criminal suspect. •Gel electrophoresis is a widely used technique for separating electrically charged molecules.
It is a central technique in molecular biology and genetic laboratories, because it lets researchers separate and purify the nucleic acids DNA and RNA and proteins, so they can be studied individually. Gel electrophoresis is often followed by staining or blotting procedures used to identify the separated molecules. What is it used for?
This is done on an automated cycler, which can heat and cool the tubes with the reaction mixture in a very short time.
This step presents the ideal temperature for the polymerase. Here, the primer (a piece of DNA that is base paired to the template strand in such a way that the 3’ end of it is available to serve as the starting point for the new DNA) starts the process of “multiplying” the amount of DNA by attracting bases to attach to it. Primers that are on positions with no exact match get loose again and don’t give an extension of the fragment The Basic Steps in Gel Electrophoresis 1. There is a permeable gel with a row of holes on one side. 2.
Different DNA samples are placed in holes using pipette. 3. An electric current is sent through the gel, with the opposite side as positive (the DNA is negatively charged).
... happen if there is a large amount of greater quality DNA, this technique is stated to be ‘laborious and difficult to automate ... live in. (David Wright, 2000: 162) Main Body The techniques for identification of DNA profiling only need just a small amount of ... of three steps denaturation, annealing and elongation. The strands get separated and bind to primers (fig 5), which are pieces of ...
4. Since the larger parts of DNA cannot get far through the gel and the smaller parts can. Several bands will be formed on the gel. 5. The bands are visible under UV light How are we going to Present? •We are going to create a PowerPoint that includes relevant information on what are the techniques, what are they used for, who invented it, etc… •We are going to include a virtual lab for PCR •We are going to include a video for each.